In vitro effect of lasertherapy and photodynamic therapy in bacterial reduction presents in root canals

Authors

  • Caroline Pietroski Grando Universidade Regional Integrada do Alto Uruguai e das Missões - Câmpus de Erechim
  • Yasmin Carla Bianchini Universidade Regional Integrada do Alto Uruguai e das Missões
  • Neiva Aparecida Grazziotin Universidade Regional Integrada do Alto Uruguai e das Missões
  • Pauline Mastella Lang Universidade Regional Integrada do Alto Uruguai e das Missões
  • Simone Tuchtenhagen Universidade Regional Integrada do Alto Uruguai e das Missões
  • Bruno Emmanuelli Universidade Regional Integrada do Alto Uruguai e das Missões

DOI:

https://doi.org/10.5335/rfo.v25i2.10645

Keywords:

Endodontics, Disinfection, Fototherapy, Photodynamic therapy

Abstract

This study aims to verify in vitro the bactericidal effect of laser therapy and photodynamic therapy with low power laser (660 nm and 808 nm), in bacteria present in the root canals.Methods: 60 Petri dishes were prepared with bacteria: 20 plates with Enterococcus faecalis, 20 plates with Staphylococcus aureus and 20 with Pseudomonas aeruginosa. At random, each group was divided into 10 subgroups (two plates each): three subgroups treated with 660nm laser therapy at doses of 150, 225 and 300J / cm², three subgroups treated with photodynamic therapy, (0.2% methylene blue and laser 660nm) in doses of 150, 225 and 300J / cm²; a subgroup treated with 808nm laser therapy at a dose of 225J / cm², a subgroup with (photodynamic therapy and 808nm laser) at a dose of 225J / cm²; a subgroup treated only with photosensitizer(FS), and an untreated (control). Those treated with laser therapy and photodynamic therapy were irradiated only once and incubated for 24 hours. The last two received no radiation. The cultures were analyzed visually to check the inhibition zone. In the groups submitted to laser therapy only, for the FS group and for the Control group, no inhibition halos were observed, since PDT was applied, with both L1 and L2, inhibition halos were observed in all studied bacterial species. It was concluded that laser therapy did not produce bactericidal and / or bacteriostatic effects, while photodynamic therapy at both wavelengths produced significant growth inhibition halos in the three studied bacteria.

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References

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Published

2021-06-01

Issue

Vol. 25 No. 2 (2020): RFO

Section

Investigação Científica

License

Licença Creative Commons
This work is licensed under aCreative Commons Atribuição-NãoComercial-SemDerivações 4.0 Internacional.

How to Cite

In vitro effect of lasertherapy and photodynamic therapy in bacterial reduction presents in root canals. (2021). Revista Da Faculdade De Odontologia - UPF, 25(2), 184-190. https://doi.org/10.5335/rfo.v25i2.10645