Effect of propolis extract and chlorhexidine digluconate on the biofilm formation by Candida albicans in acrylic resin
DOI:
https://doi.org/10.5335/rfo.v25i1.10998Keywords:
biofilm, Candida albicans, propolisAbstract
Objective: to evaluate in vitro the antimicrobial action of propolis extract (EP) and chlorhexidine digluconate (DCHX) in the formation of biofilm by C. albicans in thermopolymerized acrylic resin. Methods: the effect of EP and DCHX on C. albicans biofilms were evaluated by quantifying Colony Forming Units (UFCs) and by quantifying biomass by violet crystal and polysaccharides by safranin. For this purpose, C. albicans were pre-adhered to specimens and only on polystyrene microplates and subsequently the treatment was performed with different concentrations of EP (221 and 443 μg / mL) and DCHX (0.25% and 0,5%). Results: a significant reduction in the biofilm formation by C. albicans was demonstrated, with both tested substances and in all concentrations. Conclusion: in view of the difficulties and the various factors that interfere in the treatment of oral candidiasis with antifungals and the fact that it has been reported as a public health problem, with a high incidence in users of removable prostheses, it is necessary to launch other alternatives that may be more effective in the treatment of this infection, taking into account the side effects for the patient, with EP being well indicated, as it is a topical product and, therefore, easy to apply, having a lower cost .
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