Antimicrobial activity of intracanal dressings composed by natural products associated to chlorhexidine and its influence on dentinal colour change
DOI:
https://doi.org/10.5335/rfo.v25i1.9915Keywords:
calcium hydroxide, chlorhexidine, ginger extract, grape seed extract.Abstract
Purpose: The aim of study was to evaluate the antimicrobial activity of intracanal dressings and their influence on dentinal colour changes. Material and methods: Eighty samples were divided into eight groups (n=10) according to intracanal dressing protocols: G1–distilled water (DW); G2–2% chlorhexidine gel (CHX); G3–calcium hydroxide (Ca[OH]2)+DW; G4–grape seed extract (GSE)+DW; G5–ginger extract (GE)+DW; G6–Ca(OH)2+CHX; G7–GSE+CHX; and G8–GE+CHX. The antimicrobial activity was evaluated by colony-forming units (CFUs) counting and dentinal colour changes was evaluated by digital spectrophotometry. Data were statistically analysed by specific tests (α=0.05). Results: The highest bacterial reduction was observed in groups 4, 6, 7 and 8, with no significant difference between them (p<0.05). Groups 4 and 7 showed the highest medians of dentinal colour change (p<0.05). Conclusion: The addition of CHX improved the antimicrobial activity of GE-based intracanal dressing, with no effect in GSE-based intracanal dressing; moreover, these protocols induced significant dentinal colour changes.
Downloads
Download data is not yet available.
References
1. Kakehashi S, Stanley HR, Fitzgerald RJ. The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats. Oral Surg Oral Med Oral Pathol 1965; 20:340–349
2. George S, Kishen A, Song KP. The role of environmental changes on monospecies biofilm formation on root canal wall by Enterococcus faecalis. J Endod 2005; 31:867–872
3. Qualtrough AJE, Burke FJT. A look at dental aesthetics. Quintessence Int 1994; 25:7–14
4. Leonardo MR, Hernandez ME, Silva LA, Tanomaru-Filho M. Effect of a calcium hydroxide-based root canal dressing on periapical repair in dogs: a histological study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006; 102:680–685
5. Siqueira JF Jr, Rôças IN, Lopes HP, de Uzeda M. Coronal leakage of two root canal sealers containing calcium hydroxide after exposure to human saliva. J Endod 1999; 25:14–16.
6. Sathorn C, Parashos P, Messer H. Antibacterial efficacy of calcium hydroxide intracanal dressing: a systematic review and meta-analysis. Int Endod J 2007; 40:2–10.
7. Gomes BP, Vianna ME, Sena NT, Zaia AA, Ferraz CC, Souza-Filho FJ. In vitro evaluation of the antimicrobial activity of calcium hydroxide combined with chlorhexidine gel used as intracanal medicament. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006; 102:544–550
8. Dametto FR, Ferraz CC, Gomes BP, Zaia AA, Teixeira FB, Souza-Filho FJ. In vitro assessment of the immediate and prolonged antimicrobial action of chlorhexidine gel as an endodontic irrigant against Enterococcus faecalis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2005; 99:768–772
9. Böttcher DE, Sehnem NT, Montagner F, Fatturi Parolo CC, Grecca FS. Evaluation of the effect of Enterococcus faecalis biofilm on the 2% chlorhexidine substantivity: An in vitro study. J Endod 2015; 41:1364–70
10. Cecchin D, Farina AP, Souza MA, Albarello LL, Schneider AP, Vidal CM, Bedran-Russo AK. Evaluation of antimicrobial effectiveness and dentine mechanical properties after use of chemical and natural auxiliary irrigants. J Dent 2015; 43:695–702
11. Maekawa LE, Valera MC, Oliveira LD, Carvalho CA, Camargo CH, Jorge AO. Effect of Zingiber officinale and propolis on microorganisms and endotoxins in root canals. J Appl Oral Sci 2013; 21:25–31
12. Guerreiro-Tanomaru JM, de Faria-Júnior NB, Duarte MA, Ordinola-Zapata R, Graeff MS, Tanomaru-Filho M. Comparative analysis of Enterococcus faecalis biofilm formation on different substrates. J Endod 2013; 39:346–350
13. Peters LB, Wesselink PR, Moorer WR. The fate and the role of bacteria left in root dentinal tubules. Int Endod J 1995; 28:95–99
14. Pinheiro ET, Gomes BPFA, Ferraz CCR, Sousa ELR, Teixeira FB, Souza-Filho FJ. Microorganisms from canals of root-filled teeth with periapical lesions. Int Endod J 2003; 36:1–11
15. Figdor D, Davies JK, Sundqvist G. Starvation survival, growth and recovery of Enterococcus faecalis in human serum. Molec Oral Microb 2003; 18:234–239
16. Portenier I, Haapasalo H, Rye A, Waltino T, Orstavik D, Haapasalo M. Inactivation of root canal medicaments by dentine, hydroxylapatite and bovine serum albumin. Int Endod J 2001; 34:184–188
17. Furiga A, Roques C, Badet C. Preventive effects of an original combination of grape seed polyphenols with amine fluoride on dental biofilm formation and oxidative damage by oral bacteria. J Appl Microbiol 2014; 116:761–771
18. Aguiar TR, Vidal CM, Phansalkar RS, Todorova I, Napolitano JG, McAlpine JB, Chen SN, Pauli GF, Bedran-Russo AK. Dentin biomodification potential depends on polyphenol source. J Dental Res 2014; 93:417–422
19. Park M, Bae J, Lee DS. Antibacterial activity of [10]-gingerol and [12]-gingerol isolated from ginger rhizome against periodontal bacteria. Phytother Res 2008; 22:1446–1449
20. Lantz RC, Chen GJ, Sarihan M, Sólyom AM, Jolad SD, Timmerman BN. The effect of extracts from ginger rhizome on inflammatory mediator production. Phytomedicine 2007; 14:123–128
21. Souza M, Cecchin D, Barbizam JV, Almeida JF, Zaia AA, Gomes BP, Ferraz CC. Evaluation of the colour change in enamel and dentine promoted by the interaction between 2% chlorhexidine and auxiliary chemical solutions. Aust Endod J 2013; 39:107–111
22. Meireles SS, Santos IS, Bona AD, Demarco FF. A double-blind randomized clinical trial of two carbamide peroxide tooth bleaching agents: 2-year follow-up. J Dent 2010; 38:956–963
23. Thimothe J, Bonsi IA, Padilla-Zakour OI, Kooh H. Chemical characterization of red wine grape (Vitis vinifera and Vitis interspecific hybrids) and pomace phenolic extracts and their biological activity against Streptococcus mutans. J Agricult Food Chem 2007; 12:10200–10207
24. Perez-Heredia M, Ferrer-Luque CM, Gonzalez-Rodriguez MP, Martin-Peinado FJ, González-López S. Decalcifying effect of 15% EDTA, 15% citric acid, 5% phosphoric acid and 2.5% sodium hypochlorite on root canal dentine. Int Endod J 2008; 41:418–23.
25. Stojicic S, Shen Y, Qian W, Johnson B, Haapasalo M. Antibacterial and smear layer removal ability of a novel irrigant, QMiX. Int Endod J 2012; 45:363–371
2. George S, Kishen A, Song KP. The role of environmental changes on monospecies biofilm formation on root canal wall by Enterococcus faecalis. J Endod 2005; 31:867–872
3. Qualtrough AJE, Burke FJT. A look at dental aesthetics. Quintessence Int 1994; 25:7–14
4. Leonardo MR, Hernandez ME, Silva LA, Tanomaru-Filho M. Effect of a calcium hydroxide-based root canal dressing on periapical repair in dogs: a histological study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006; 102:680–685
5. Siqueira JF Jr, Rôças IN, Lopes HP, de Uzeda M. Coronal leakage of two root canal sealers containing calcium hydroxide after exposure to human saliva. J Endod 1999; 25:14–16.
6. Sathorn C, Parashos P, Messer H. Antibacterial efficacy of calcium hydroxide intracanal dressing: a systematic review and meta-analysis. Int Endod J 2007; 40:2–10.
7. Gomes BP, Vianna ME, Sena NT, Zaia AA, Ferraz CC, Souza-Filho FJ. In vitro evaluation of the antimicrobial activity of calcium hydroxide combined with chlorhexidine gel used as intracanal medicament. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006; 102:544–550
8. Dametto FR, Ferraz CC, Gomes BP, Zaia AA, Teixeira FB, Souza-Filho FJ. In vitro assessment of the immediate and prolonged antimicrobial action of chlorhexidine gel as an endodontic irrigant against Enterococcus faecalis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2005; 99:768–772
9. Böttcher DE, Sehnem NT, Montagner F, Fatturi Parolo CC, Grecca FS. Evaluation of the effect of Enterococcus faecalis biofilm on the 2% chlorhexidine substantivity: An in vitro study. J Endod 2015; 41:1364–70
10. Cecchin D, Farina AP, Souza MA, Albarello LL, Schneider AP, Vidal CM, Bedran-Russo AK. Evaluation of antimicrobial effectiveness and dentine mechanical properties after use of chemical and natural auxiliary irrigants. J Dent 2015; 43:695–702
11. Maekawa LE, Valera MC, Oliveira LD, Carvalho CA, Camargo CH, Jorge AO. Effect of Zingiber officinale and propolis on microorganisms and endotoxins in root canals. J Appl Oral Sci 2013; 21:25–31
12. Guerreiro-Tanomaru JM, de Faria-Júnior NB, Duarte MA, Ordinola-Zapata R, Graeff MS, Tanomaru-Filho M. Comparative analysis of Enterococcus faecalis biofilm formation on different substrates. J Endod 2013; 39:346–350
13. Peters LB, Wesselink PR, Moorer WR. The fate and the role of bacteria left in root dentinal tubules. Int Endod J 1995; 28:95–99
14. Pinheiro ET, Gomes BPFA, Ferraz CCR, Sousa ELR, Teixeira FB, Souza-Filho FJ. Microorganisms from canals of root-filled teeth with periapical lesions. Int Endod J 2003; 36:1–11
15. Figdor D, Davies JK, Sundqvist G. Starvation survival, growth and recovery of Enterococcus faecalis in human serum. Molec Oral Microb 2003; 18:234–239
16. Portenier I, Haapasalo H, Rye A, Waltino T, Orstavik D, Haapasalo M. Inactivation of root canal medicaments by dentine, hydroxylapatite and bovine serum albumin. Int Endod J 2001; 34:184–188
17. Furiga A, Roques C, Badet C. Preventive effects of an original combination of grape seed polyphenols with amine fluoride on dental biofilm formation and oxidative damage by oral bacteria. J Appl Microbiol 2014; 116:761–771
18. Aguiar TR, Vidal CM, Phansalkar RS, Todorova I, Napolitano JG, McAlpine JB, Chen SN, Pauli GF, Bedran-Russo AK. Dentin biomodification potential depends on polyphenol source. J Dental Res 2014; 93:417–422
19. Park M, Bae J, Lee DS. Antibacterial activity of [10]-gingerol and [12]-gingerol isolated from ginger rhizome against periodontal bacteria. Phytother Res 2008; 22:1446–1449
20. Lantz RC, Chen GJ, Sarihan M, Sólyom AM, Jolad SD, Timmerman BN. The effect of extracts from ginger rhizome on inflammatory mediator production. Phytomedicine 2007; 14:123–128
21. Souza M, Cecchin D, Barbizam JV, Almeida JF, Zaia AA, Gomes BP, Ferraz CC. Evaluation of the colour change in enamel and dentine promoted by the interaction between 2% chlorhexidine and auxiliary chemical solutions. Aust Endod J 2013; 39:107–111
22. Meireles SS, Santos IS, Bona AD, Demarco FF. A double-blind randomized clinical trial of two carbamide peroxide tooth bleaching agents: 2-year follow-up. J Dent 2010; 38:956–963
23. Thimothe J, Bonsi IA, Padilla-Zakour OI, Kooh H. Chemical characterization of red wine grape (Vitis vinifera and Vitis interspecific hybrids) and pomace phenolic extracts and their biological activity against Streptococcus mutans. J Agricult Food Chem 2007; 12:10200–10207
24. Perez-Heredia M, Ferrer-Luque CM, Gonzalez-Rodriguez MP, Martin-Peinado FJ, González-López S. Decalcifying effect of 15% EDTA, 15% citric acid, 5% phosphoric acid and 2.5% sodium hypochlorite on root canal dentine. Int Endod J 2008; 41:418–23.
25. Stojicic S, Shen Y, Qian W, Johnson B, Haapasalo M. Antibacterial and smear layer removal ability of a novel irrigant, QMiX. Int Endod J 2012; 45:363–371
Downloads
Published
2020-12-11
Issue
Section
Investigação Científica
License
This work is licensed under aCreative Commons Atribuição-NãoComercial-SemDerivações 4.0 Internacional.
How to Cite
Antimicrobial activity of intracanal dressings composed by natural products associated to chlorhexidine and its influence on dentinal colour change. (2020). Revista Da Faculdade De Odontologia - UPF, 25(1), 42-49. https://doi.org/10.5335/rfo.v25i1.9915
